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Literature summary extracted from

  • Roberts, G.; Cooper, L.; White, J.; Su, T.; Zipprich, J.; Geary, P.; Kennedy, C.; Dryden, D.
    An investigation of the structural requirements for ATP hydrolysis and DNA cleavage by the EcoKI type I DNA restriction and modification enzyme (2011), Nucleic Acids Res., 39, 7667-7676.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.11.5 overexpression of RecBCD in Escherichia coli strain SCK387 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.11.5 Mg2+ activates Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.5 Escherichia coli
-
-
-
3.1.11.5 Escherichia coli JM109
-
-
-

Synonyms

EC Number Synonyms Comment Organism
3.1.11.5 RecBCD exonuclease
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.11.5 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.11.5 7.5
-
 assay at Escherichia coli

General Information

EC Number General Information Comment Organism
3.1.11.5 physiological function type I DNA restriction/modification systems are oligomeric enzymes capable of switching between a methyltransferase function on hemimethylated host DNA and an endonuclease function on unmethylated foreign DNA. Reuse of the methyltransferase subunits is possible so that restriction proceeds until the restriction subunits are depleted. RecBCD exonuclease halts restriction and does not assist recycling, influence of RecBCD on the EcoKI type I restriction enzyme, overview Escherichia coli